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Image Search Results
Journal: Human Gene Therapy
Article Title: A Short Polypeptide from the Herpes Simplex Virus Type 2 ICP10 Gene Can Induce Antigen Aggregation and Autophagosomal Degradation for Enhanced Immune Presentation
doi: 10.1089/hum.2010.080
Figure Lengend Snippet: ELISPOT assays of immune responses to class I and II OVA peptides. Mice were vaccinated with the indicated constructs twice before spleens were taken for ELISPOT assays as described in Materials and Methods. (A) Quantification of IFN-γ ELISPOT from purified CD4+ T cells after stimulation with class II OVA peptide. (B) Results of quantification of IFN-γ ELISPOT from purified splenocytes after stimulation with class I OVA peptide. Star, p < 0.01 as compared with vaccinations of B16 or B16-pOVA; cross, p < 0.05 as compared with vaccination of B16.
Article Snippet: To separate CD4 + cells, Ficoll-purified spleen cells were passed through
Techniques: Enzyme-linked Immunospot, Construct, Purification